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Quantitative studies of rhubarb using quantitative analysis of multicomponents by single marker and response surface methodology
Author(s) -
Sun Jiachen,
Wu Yueting,
Dong Shengjie,
Li Xia,
Gao Wenyuan
Publication year - 2017
Publication title -
journal of separation science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.72
H-Index - 102
eISSN - 1615-9314
pISSN - 1615-9306
DOI - 10.1002/jssc.201700513
Subject(s) - quantitative analysis (chemistry) , chromatography , response surface methodology , chemistry
In this work, we developed a novel approach to evaluate the contents of bioactive components in rhubarb. The present method was based on the quantitative analysis of multicomponents by a single‐marker and response surface methodology approaches. The quantitative analysis of multicomponents by a single‐marker method based on high‐performance liquid chromatography coupled with photodiode array detection was developed and applied to determine the contents of 12 bioactive components in rhubarb. No significant differences were found in the results from the quantitative analysis of multicomponents by a single‐marker and the external standard method. In order to maximize the extraction of 12 bioactive compounds in rhubarb, the ultrasonic‐assisted extraction conditions were obtained by the response surface methodology coupled with Box–Behnken design. According to the obtained results, we showed that the optimal conditions would be as follows: proportion of ethanol/water 74.39%, solvent‐to‐solid ratio 24.07:1 v/w, extraction time 51.13 min, and extraction temperature 63.61°C. The analytical scheme established in this research should be a reliable, convenient, and appropriate method for quantitative determination of bioactive compounds in rhubarb.