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Strategy for the separation of concentrated samples by capillary electrophoresis
Author(s) -
Liang Shuang,
Fu Xia,
Xiao Hongting,
Li Tianxiang,
Xu Jun,
Zhang Yong
Publication year - 2017
Publication title -
journal of separation science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.72
H-Index - 102
eISSN - 1615-9314
pISSN - 1615-9306
DOI - 10.1002/jssc.201700512
Subject(s) - chromatography , capillary electrophoresis , chemistry , dispersion (optics) , electrophoresis , bovine serum albumin , capillary action , lysozyme , analytical chemistry (journal) , separation (statistics) , materials science , composite material , optics , biochemistry , physics , machine learning , computer science
The use of concentrated samples is usually avoided during conventional separations since utilization of concentrated samples normally compromises the quality of separation. However, in case of the detection of low‐abundance components, highly concentrated samples are necessary, which leads to an extremely high concentration for high‐abundant components. This will make the separation difficult due to the serious longitudinal dispersion. Here, we developed a method to separate high concentration of components based on the modified capillary electrophoresis. The mechanism involves concentrated sample stretched into a wider zone in the higher electric field strength; the sample zone is fractionated into thin sections via a cutting effect; these thin sections are then separated. Based on this mechanism, we examined to separate an overloaded mixture of N , N ′‐diphenylguanidine and N , N ′‐di( o ‐tolyl)guanidine. Baseline separation was achieved due to much small longitudinal dispersion. The theoretical plate numbers of peaks were around 3.5 × 10 5 m −1 . The practicality of the new approach is demonstrated in the separation of a model protein mixture, containing lysozyme, bovine serum albumin, and ribonuclease A.