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Vortex‐ultrasound‐assisted dispersive liquid–liquid microextraction coupled with gas chromatography–mass spectrometry for the analysis of volatile bioactive components and comparative pharmacokinetic study of the herb–herb interactions in Guanxin Shutong Capsule
Author(s) -
Mu Jingqing,
Gao Xun,
Li Qing,
Yang Xiaomei,
Yang Wenling,
Sun Xu,
Bi Kaishun,
Zhang Huifen
Publication year - 2017
Publication title -
journal of separation science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.72
H-Index - 102
eISSN - 1615-9314
pISSN - 1615-9306
DOI - 10.1002/jssc.201700500
Subject(s) - borneol , chemistry , chromatography , pharmacokinetics , mass spectrometry , herb , gas chromatography–mass spectrometry , capsule , camphor , gas chromatography , medicinal herbs , traditional medicine , pharmacology , traditional chinese medicine , medicine , botany , alternative medicine , organic chemistry , pathology , biology
Guanxin Shutong Capsule, an effective traditional Chinese medicine, is widely used for coronary heart disease clinically. Volatile components are one of its important bioactive constituents. To better understand the material basis for the therapeutic effects, the components of Guanxin Shutong Capsule absorbed into the blood and their metabolites were identified based on gas chromatography with mass spectrometry coupled with vortex‐ultrasound‐assisted dispersive liquid–liquid microextraction. As a result, three prototypes and 15 metabolites were identified or tentatively characterized in rat plasma. Subsequently, a pharmacokinetic study was carried out to monitor the concentrations of the main bioactive constituents and metabolites (isoborneol, borneol, eugenol, and camphor) by gas chromatography with mass spectrometry in rat plasma following oral administration of single herb extract and different combinations of herbs in this prescription. Compared to other groups, a statistically significant difference of the pharmacokinetic properties was obtained when the total complex prescription was administered, indicating possible drug–drug interactions among the complex ingredients of Guanxin Shutong Capsule. These findings provided an experimental basis concerning the clinical application and medicinal efficacy of Guanxin Shutong Capsule in the treatment of coronary heart disease.