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Postcolumn determination of polyphenolic antioxidants in Cirsium vulgare (Savi) Ten. extracts
Author(s) -
NalewajkoSieliwoniuk Edyta,
Malejko Julita,
Twarowska Paulina,
Timoszuk Magdalena,
Nazaruk Jolanta
Publication year - 2017
Publication title -
journal of separation science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.72
H-Index - 102
eISSN - 1615-9314
pISSN - 1615-9306
DOI - 10.1002/jssc.201700496
Subject(s) - chemistry , polyphenol , chromatography , chlorogenic acid , luteolin , luminol , apigenin , rutin , chemiluminescence , antioxidant , flavonoid , biochemistry
Novel methods for the determination of polyphenolic antioxidants present in extracts from inflorescences of Cirsium vulgare (Savi) Ten. based on ultra‐high performance liquid chromatography with photodiode array and chemiluminescence detection have been developed. Under the optimized conditions of chromatographic separation the analytical characteristic of the method was performed. The proposed method was successfully applied to the determination of ten polyphenols present in inflorescences of Cirsium vulgare . A comparison of the contents of analytes in extracts prepared by using various extraction media (methanol, ethanol, 70% methanol, 70% ethanol, and water) was carried out for the first time. For the postcolumn detection of scavenging activity of polyphenolic antioxidants against reactive oxygen species (H 2 O 2 , • OH, O 2 • − ) three systems based on chemiluminescence of luminol were used. A review of the current scientific literature shows that this is the first report on the application of luminol‐based postcolumn detection for the on‐line investigation of • OH scavenging activity. The main compound determined in extracts from inflorescences of Cirsium vulgare was apigenin 7‐ O ‐glucuronide, whereas the highest antioxidant activity was observed for chlorogenic acid, luteolin 7‐ O ‐glucoside, and apigenin.