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Fingerprint profiles of flavonoid compounds from different Psidium guajava leaves and their antioxidant activities
Author(s) -
Wang Lu,
Wu Yanan,
Bei Qi,
Shi Kan,
Wu Zhenqiang
Publication year - 2017
Publication title -
journal of separation science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.72
H-Index - 102
eISSN - 1615-9314
pISSN - 1615-9306
DOI - 10.1002/jssc.201700477
Subject(s) - psidium , chemistry , quercitrin , flavonoid , quercetin , chromatography , rutin , kaempferol , dpph , glycoside , antioxidant , food science , botany , biochemistry , organic chemistry , biology
Flavonoids are the main active components in Psidium guajava leaves and have many multi‐physiological functions. In this study, the flavonoid compositions were identified in the Psidium guajava leaves samples using a high‐performance liquid chromatography with time‐of‐flight electrospray ionization mass spectrometry method. A high‐performance liquid chromatography fingerprint method, combined with chemometrics, was used to perform a quality assessment of the Psidium guajava leaves samples. The eight identified flavonoid compounds including rutin, isoquercitrin, quercetin‐3‐ O ‐β‐ d ‐xylopyranoside, quercetin‐3‐ O ‐α‐ l ‐arabinopyranoside, avicularin, quercitrin, quercetin, and kaempferol were used as the chemical markers. The antioxidant activity of 15 batches of samples was examined using three different methods, and the results revealed the Psidium guajava leaves samples that had higher contents of the flavonoid compounds, glycoside and aglycone, possessed the highest antioxidant capacities. Consequently, a combination of chromatographic fingerprints and chemometric analyses was used for a quality assessment of Psidium guajava leaf tea and its derived products, which can lay the foundation for the development of plant tea resources or other herbs.