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Determination of polychlorinated dibenzo‐ p ‐dioxins and polychlorinated dibenzofurans, and dioxin‐like polychlorinated biphenyls in human serum using programmable‐temperature vaporization gas chromatography with high‐resolution mass spectrometry
Author(s) -
Zhang Lei,
Zhong Yuxin,
Liu Xin,
Bao Yan,
Zhao Yunfeng,
Wu Yongning,
Cai Zongwei,
Li Jingguang
Publication year - 2017
Publication title -
journal of separation science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.72
H-Index - 102
eISSN - 1615-9314
pISSN - 1615-9306
DOI - 10.1002/jssc.201700465
Subject(s) - polychlorinated dibenzofurans , chemistry , vaporization , gas chromatography , environmental chemistry , detection limit , chromatography , population , mass spectrometry , polychlorinated dibenzo p dioxins , pollutant , organic chemistry , demography , sociology
The determination of polychlorinated dibenzo‐ p ‐dioxins and polychlorinated dibenzofurans, and dioxin‐like polychlorinated biphenyls in blood from a non‐occupational population is essential for the investigation of adverse health effects from these pollutants. In this study, a sensitive method based on programmable‐temperature vaporization with large‐volume injection coupled with gas chromatography with high‐resolution mass spectrometry was developed to determine these pollutants in 1–2 mL of human serum samples. Various key parameters of programmable‐temperature vaporization injector, including vent temperature, vent time, vent flow, transfer temperature and transfer time were optimized by factorial design. The accuracy and precision as well as applicability were assessed by determining polychlorinated dibenzo‐ p ‐dioxins, polychlorinated dibenzofurans, and dioxin‐like polychlorinated biphenyls in calibration standard solutions, standard reference materials and real human serum samples from non‐occupational population. The method detection limits of polychlorinated dibenzo‐ p ‐dioxins and polychlorinated dibenzofurans, and dioxin‐like polychlorinated biphenyls were 1.5–9.0 and 0.005–0.02 ng/kg wet weight, respectively. By comparing with typically splitless injection, the application of programmable‐temperature vaporization injector could effectively lead to higher detectable rate of polychlorinated dibenzo‐ p ‐dioxins and polychlorinated dibenzofurans, and dioxin‐like polychlorinated biphenyls in 1–2 mL of human serum samples.