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Dispersive micro‐solid‐phase extraction using carbon‐based adsorbents for the sensitive determination of verapamil in plasma samples coupled with capillary electrophoresis
Author(s) -
Jouyban Abolghasem,
Hamidi Samin
Publication year - 2017
Publication title -
journal of separation science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.72
H-Index - 102
eISSN - 1615-9314
pISSN - 1615-9306
DOI - 10.1002/jssc.201700385
Subject(s) - capillary electrophoresis , solid phase extraction , chromatography , extraction (chemistry) , graphene , adsorption , chemistry , coprecipitation , materials science , analytical chemistry (journal) , nanotechnology , inorganic chemistry , organic chemistry
A dispersive micro‐solid‐phase extraction procedure coupled with capillary electrophoresis ultraviolet detection was developed for determination of verapamil in plasma samples. Graphene oxide/polydopamin was synthesized by a one‐step polymerization method, and graphene oxide/Fe 3 O 4 (magnetic graphene oxide) nanocomposite was prepared by coprecipitation method. Moreover, they were fully characterized. The use of hazardous and water‐immiscible solvents was scaled down, and only 500 μL of acetone was required as the desorption solvent. The detector response concentration plots were linear in the range of 5–500 ng/mL, and the proposed method was validated according to guidelines. The precision and accuracy were less than 15%. Dispersive micro‐solid‐phase extraction method provides a rapid, environmentally friendly, and sensitive analysis for the verapamil in patient plasma samples, which is adequate for therapeutic drug monitoring and pharmacokinetic studies.