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A flow‐through column electrolytic cell for supercritical fluid chromatography *
Author(s) -
Yamamoto Kazuhiro,
Ueki Tatsuya,
Higuchi Naoyuki,
Takahashi Kouji,
Kotani Akira,
Hakamata Hideki
Publication year - 2017
Publication title -
journal of separation science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.72
H-Index - 102
eISSN - 1615-9314
pISSN - 1615-9306
DOI - 10.1002/jssc.201700317
Subject(s) - supercritical fluid chromatography , ferrocene , chromatography , elution , electrolyte , supercritical fluid , electrode , analytical chemistry (journal) , chemistry , column chromatography , supporting electrolyte , working electrode , detection limit , materials science , electrochemistry , organic chemistry
A novel flow‐through column electrolytic cell was proposed as a detector to obtain current signals for supercritical fluid chromatography. The electrochemical cell consisted of two electrodes and its holder, and a working and a counter electrode were fabricated from 192 carbon strings, which were composed of 400 carbon fibers of 10 μm in diameter filled into a heat‐shrinkable tube. These electrodes were placed in the center of a holder made from polyether ether ketone blocks and they were separated by polytetrafluoroethylene membrane filters. To evaluate the sensitivity of this cell, a standard solution of ferrocene was injected into the supercritical fluid chromatography system connected to the electrolytic cell. The ferrocene was eluted through a silica gel column using a mixture of a mobile phase of supercritical CO 2 and a modifier of methanol containing ammonium acetate. The current peak area of ferrocene correlated to the ferrocene concentration in the range of 10–400 μmol/L ( r = 0.999). Moreover, the limit of detection on the column estimated from a signal‐to‐noise ratio of 3 was 9.8 × 10 −13 mol.