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Simultaneous determination of pyrametostrobin and its two metabolites in cucumber and soil using a quick, easy, cheap, effective, rugged, and safe method with HPLC–MS/MS
Author(s) -
Huang Chengtian,
Zhu He,
Yu Yang,
Ding Qi,
Jia Fuyan,
Zhao Bochui,
Wei Siyuan,
Gong Dangsheng,
Xu Weichang,
Ji Mingshan
Publication year - 2017
Publication title -
journal of separation science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.72
H-Index - 102
eISSN - 1615-9314
pISSN - 1615-9306
DOI - 10.1002/jssc.201700109
Subject(s) - chromatography , chemistry , amine gas treating , extraction (chemistry) , electrospray ionization , mass spectrometry , high performance liquid chromatography , matrix (chemical analysis) , acetonitrile , solvent , sample preparation , tandem mass spectrometry , organic chemistry
An easy, effective and sensitive analytical method for the simultaneous determination of a novel fungicide pyrametostrobin and its two metabolites pyrametostrobin‐M1 and pyrametostrobin‐M2 in cucumber and soil was developed using a quick, easy, cheap, effective, rugged, and safe method with high‐performance liquid chromatography and tandem mass spectrometry. The extraction solvent was acetonitrile, and cleanup sorbents were primary secondary amine and graphitized carbon black for cucumber samples and primary secondary amine for soil samples. The three target compounds were successfully separated between 3.2 and 3.9 min using a Waters CORTECS™ C 18 column connected to an electrospray ionization source. All the matrix‐matched samples at three fortified levels (10, 100 and 1000 μg/kg) provided satisfactory recoveries in the range of 78.8–93.8% with relative standard deviations below 6.9%. The limits of quantitation for the three compounds were below 0.183 μg/kg. The proposed method was validated by analyzing real samples.