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Simultaneous quantitation of 14 active components in Yinchenhao decoction with an ultrahigh performance liquid chromatography‐diode array detector: Method development and ingredient analysis of different commonly prepared samples
Author(s) -
Yi YaXiong,
Zhang Yong,
Ding Yue,
Lu Lu,
Zhang Tong,
Zhao Yuan,
Xu XiaoJun,
Zhang YuXin
Publication year - 2016
Publication title -
journal of separation science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.72
H-Index - 102
eISSN - 1615-9314
pISSN - 1615-9306
DOI - 10.1002/jssc.201670211
Subject(s) - decoction , chromatography , chromatography detector , active ingredient , ingredient , high performance liquid chromatography , chemistry , traditional medicine , medicine , food science , pharmacology
J. Sep. Sci . 2016, 39 , 4147–4157 DOI: 10.1002/jssc.201600284 Yinchenhao decoction (YCHD) is a famous Chinese herbal formula recorded in the Shang Han Lun which was prescribed by Zhongjing Zhang during 150–219 AD. A novel quantitative analysis method was developed, based on ultrahigh performance liquid chromatography coupled with a diode array detector for the simultaneous determination of 14 main active components in Yinchenhao decoction. Furthermore, the method has been applied for compositional difference analysis of the 14 components in eight normal extraction samples of Yinchenhao decoction, with the aid of hierarchical clustering analysis and similarity analysis. The present research could help hospital, factory and lab choose the best way to make Yinchenhao decoction with better efficacy.