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Effective length calibration method for processing the fluorescence signal detected by charge‐coupled device in capillary electrophoresis
Author(s) -
Ni Yi,
Liu Chenchen,
Chen Jin,
Chen Qinmiao,
Zhu Xifang,
Dou Xiaoming
Publication year - 2017
Publication title -
journal of separation science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.72
H-Index - 102
eISSN - 1615-9314
pISSN - 1615-9306
DOI - 10.1002/jssc.201601458
Subject(s) - electropherogram , capillary electrophoresis , detector , photomultiplier , calibration , signal (programming language) , charge coupled device , pixel , optics , electrophoresis , noise (video) , materials science , analytical chemistry (journal) , chemistry , physics , chromatography , computer science , artificial intelligence , quantum mechanics , image (mathematics) , programming language
A novel method named effective length calibration method has been developed to process the fluorescence signal detected by charge‐coupled device during capillary electrophoresis. The new method treated each pixel as an individual point detector, and effectively binned a large number of pixels into a final electropherogram without losing the narrow detection window defined by a single pixel. Capillary electrophoresis separations of DNA were carried out and detected by charge‐coupled device and conventional detector (photomultiplier tube). Detection properties including signal‐to‐noise ratio, peak width, detection frequency, and tilt of detector were investigated. It was found that the new method achieved much higher signal‐to‐noise ratio and smaller peak width than the conventional detector did. A Detection width of 0.5 μm was easily achieved.