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Simple, rapid, and environmentally friendly method for the separation of isoflavones using ultra‐high performance supercritical fluid chromatography
Author(s) -
Wu Wenjie,
Zhang Yuan,
Wu Hanqiu,
Zhou Weie,
Cheng Yan,
Li Hongna,
Zhang Chuanbin,
Li Lulu,
Huang Ying,
Zhang Feng
Publication year - 2017
Publication title -
journal of separation science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.72
H-Index - 102
eISSN - 1615-9314
pISSN - 1615-9306
DOI - 10.1002/jssc.201601454
Subject(s) - chromatography , isoflavones , chemistry , supercritical fluid chromatography , formic acid , supercritical fluid , elution , high performance liquid chromatography , analyte , organic chemistry , biochemistry
Isoflavones are natural substances that exhibit hormone‐like pharmacological activities. The separation of isoflavones remains an analytical challenge because of their similar structures. We show that ultra‐high performance supercritical fluid chromatography can be an appropriate tool to achieve the fast separation of 12 common dietary isoflavones. Among the five tested columns the Torus DEA column was found to be the most effective column for the separation of these isoflavones. The impact of individual parameters on the retention time and separation factor was evaluated. These parameters were optimized to develop a simple, rapid, and green method for the separation of the 12 target analytes. It only took 12.91 min using gradient elution with methanol as an organic modifier and formic acid as an additive. These isoflavones were determined with limit of quantitation ranging from 0.10 to 0.50 μg/mL, which was sufficient for reliable determination of various matrixes.