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Ionic liquids based on imidazole for online concentration of catecholamines in capillary electrophoresis
Author(s) -
Bessonova Elena,
Kartsova Liudmila,
Gallyamova Valeria
Publication year - 2017
Publication title -
journal of separation science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.72
H-Index - 102
eISSN - 1615-9314
pISSN - 1615-9306
DOI - 10.1002/jssc.201601394
Subject(s) - capillary electrophoresis , chemistry , electrokinetic phenomena , chromatography , analyte , micellar electrokinetic chromatography , imidazole , stacking , ionic liquid , isotachophoresis , analytical chemistry (journal) , chloride , detection limit , electrophoresis , sample preparation , matrix (chemical analysis) , electrolyte , organic chemistry , electrode , catalysis
Potential possibilities of long‐chain ionic liquids based on imidazole (1‐dodecyl‐3‐methylimidazolium chloride and 1‐cetyl‐3‐methylimidazolium chloride) for online sample concentration techniques (field‐amplified sample stacking, head‐column field‐amplified sample stacking, and sweeping) of catecholamines were studied in both capillary zone electrophoresis and micellar electrokinetic chromatography. The use of a high‐conductivity sample matrix in sweeping was found to significantly increase the separation efficiency of analyte up to 2 × 10 6 theoretical plates per meter and remarkably reduce limits of detection for catecholamines up to 50 ng/mL. This approach was shown to be suitable for the determination of trace amounts of catecholamines in biological fluids.