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Fast and simultaneous determination of 12 polyphenols in apple peel and pulp by using chemometrics‐assisted high‐performance liquid chromatography with diode array detection
Author(s) -
Wang Tong,
Wu HaiLong,
Xie LiXia,
Zhu Li,
Liu Zhi,
Sun XiaoDong,
Xiao Rong,
Yu RuQin
Publication year - 2017
Publication title -
journal of separation science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.72
H-Index - 102
eISSN - 1615-9314
pISSN - 1615-9306
DOI - 10.1002/jssc.201601293
Subject(s) - chemometrics , chromatography , detection limit , analyte , chemistry , polyphenol , high performance liquid chromatography , elution , pulp (tooth) , partial least squares regression , analytical chemistry (journal) , computer science , machine learning , biochemistry , antioxidant , medicine , pathology
In this work, a smart chemometrics‐enhanced strategy, high‐performance liquid chromatography, and diode array detection coupled with second‐order calibration method based on alternating trilinear decomposition algorithm was proposed to simultaneously quantify 12 polyphenols in different kinds of apple peel and pulp samples. The proposed strategy proved to be a powerful tool to solve the problems of coelution, unknown interferences, and chromatographic shifts in the process of high‐performance liquid chromatography analysis, making it possible for the determination of 12 polyphenols in complex apple matrices within 10 min under simple conditions of elution. The average recoveries with standard deviations, and figures of merit including sensitivity, selectivity, limit of detection, and limit of quantitation were calculated to validate the accuracy of the proposed method. Compared to the quantitative analysis results from the classic high‐performance liquid chromatography method, the statistical and graphical analysis showed that our proposed strategy obtained more reliable results. All results indicated that our proposed method used in the quantitative analysis of apple polyphenols was an accurate, fast, universal, simple, and green one, and it was expected to be developed as an attractive alternative method for simultaneous determination of multitargeted analytes in complex matrices.