Premium
Poly(methacrylic acid‐ethylene glycol dimethacrylate‐ N ‐vinylcarbazole) monolithic column for the enrichment of trace benzodiazepines from urine and beer samples
Author(s) -
Yao Weixuan,
Fan Zhefeng,
Zhang Suling
Publication year - 2017
Publication title -
journal of separation science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.72
H-Index - 102
eISSN - 1615-9314
pISSN - 1615-9306
DOI - 10.1002/jssc.201601263
Subject(s) - methacrylic acid , chemistry , ethylene glycol , chromatography , ethylene glycol dimethacrylate , monolithic hplc column , methacrylate , ethylene , high performance liquid chromatography , organic chemistry , polymer , copolymer , catalysis
A sensitive microextraction method based on a new poly(methacrylic acid‐ethylene glycol dimethacrylate‐ N ‐vinylcarbazole) monolithic capillary column, coupled with gas chromatography and electron capture detection, was established for the determination of three benzodiazepines (estazolam, alprazolam, and triazolam) in urine and beer samples. Owing to the abundant π electrons and polar surface of N ‐vinylcarbazole, N ‐vinylcarbazole‐incorporated monolith showed a higher extraction performance than neat poly(methacrylic acid‐ethylene glycol dimethacrylate) because of the enhanced π–π stacking interactions derived from the π‐electron‐rich benzene groups from N ‐vinylcarbazole. The monolith exhibited a homogeneous and continuous structure, good permeability, and a long lifetime. Factors affecting the extraction such as solution pH, salt concentration, sample volume, desorption solvent, and desorption volume were investigated. Under the optimized conditions, limits of detection of 0.011–0.026 ng/mL were obtained. The one‐column and column‐to‐column precision values were ≤7.2 and ≤9.8%, respectively. The real samples were first diluted with deionized water and then treated by the monolith microextraction before gas chromatography analysis. The recoveries were 81.4–93.3 and 83.3–94.7% for the spiked samples, with relative standard deviations of 4.1–8.1 and 3.8–8.5%, respectively. This method provides an accurate, simple, and sensitive detection platform for drug analysis.