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Multilayered particle‐packed column: Evaluation and comparison with monolithic and core–shell particle columns for the determination of red azo dyes in Sequential Injection Chromatography
Author(s) -
Chocholouš Petr,
Gil Renato,
Acebal Carolina C.,
Kubala Viktor,
Šatínský Dalibor,
Solich Petr
Publication year - 2017
Publication title -
journal of separation science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.72
H-Index - 102
eISSN - 1615-9314
pISSN - 1615-9306
DOI - 10.1002/jssc.201601224
Subject(s) - chromatography , acetonitrile , chemistry , elution , particle (ecology) , sorbent , high performance liquid chromatography , monolithic hplc column , particle size , analytical chemistry (journal) , column chromatography , adsorption , organic chemistry , oceanography , geology
A recently presented new type of “multilayered” organic–inorganic hybrid silica particle packed column YMC‐Triart C 18 (50 mm × 4.6 mm, 5 μm) was used for the development of a sequential injection chromatography method for determination of five azo dyes (Sudan I, Sudan II, Sudan III, Sudan orange G, and para red) in selected food seasonings. The use of a novel sorbent brings attractive features, reduced backpressure, and broader chemical stability together with high separation performance, which are discussed and compared with that of three types of columns typically used in medium‐pressure flow chromatography techniques (classic monolithic, narrow monolithic, and core–shell particle columns). The separation was performed in gradient elution mode created by the zone mixing of two mobile phases (acetonitrile/water 90:10, 1.5 mL + acetonitrile/water 100:0, 2.3 mL) at a flow rate of 0.60 mL/min and time of analysis <9.5 min. The spectrophotometric detection wavelengths were set to 400, 480, and 500 nm. The high performance of the developed method with multilayered particle column was well documented and the results indicate a broad capability of sequential injection chromatography.