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Brompheniramine as a novel probe for indirect UV detection and its application for the capillary electrophoresis of adamantane drugs
Author(s) -
Prapatpong Pornpan,
Prutthiwanasan Brompoj,
Nuchtavorn Nantana,
Buranaphalin Sawanya,
Suntornsuk Leena
Publication year - 2017
Publication title -
journal of separation science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.72
H-Index - 102
eISSN - 1615-9314
pISSN - 1615-9306
DOI - 10.1002/jssc.201601162
Subject(s) - chemistry , capillary electrophoresis , adamantane , analyte , rimantadine , derivatization , chromatography , electrolyte , detection limit , potentiometric titration , analytical chemistry (journal) , mass spectrometry , ion , electrode , organic chemistry , virus , virology , influenza a virus , biology
Brompheniramine, an antihistamine drug, was employed as a novel UV probe for capillary electrophoresis with indirect UV detection of adamantane drugs (memantine, amantadine, and rimantadine). The probe possesses high molar absorptivity of 24 × 10 3 L/mol cm at 6 mM, which enables the measurement of these nonchromophore analytes without derivatization. The simple background electrolyte (10 mM sodium dihydrogen phosphate (pH 5.0) containing 5 mM brompheniramine and 6 mM β‐cyclodextrin) provided the separation of the analytes in a short time (7.5 min). Under these conditions, brompheniramine had similar mobility to that of the analyte ions resulting in symmetric peaks with minimal electrodispersion. The analytes displace the probe at a one‐to‐one ratio with transfer values close to unity. β‐Cyclodextrin played a role in the resolution of the structurally similar adamantane derivatives. Method validation showed good linearity ( r 2 > 0.98), precision (%RSD ≤ 3.30), and accuracy (recoveries ranging from 98 to 109%). The proposed method was successfully applied to determine the adamantane content in pharmaceutical products.