Premium
Simultaneous analysis of bisphenol A based compounds and other monomers leaching from resin‐based dental materials by UHPLC–MS/MS
Author(s) -
Putzeys Eveline,
Cokic Stevan M.,
Chong Hui,
Smet Mario,
Vanoirbeek Jeroen,
Godderis Lode,
Meerbeek Bart,
Landuyt Kirsten L.,
Duca Radu Corneliu
Publication year - 2017
Publication title -
journal of separation science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.72
H-Index - 102
eISSN - 1615-9314
pISSN - 1615-9306
DOI - 10.1002/jssc.201601153
Subject(s) - chromatography , chemistry , mass spectrometry , triethylene glycol , detection limit , bisphenol a , liquid chromatography–mass spectrometry , repeatability , leaching (pedology) , sample preparation , bisphenol , epoxy , organic chemistry , environmental science , soil science , soil water
Resin‐based dental materials have raised debates concerning their safety and biocompatibility, resulting in a growing necessity of profound knowledge on the quantity of released compounds into the oral cavity. In this context, the aim of this study was to develop a comprehensive and reliable procedure based on liquid chromatography with mass spectrometry for the simultaneous analysis of various leached compounds (including bisphenol A based compounds) in samples from in vitro experiments. Different experiments were performed to determine the optimal analytical parameters, comprising mass spectrometry parameters, chromatographic separation conditions, and sample preparation. Four internal standards were used as follows: deuterated diethyl phthalate and bisphenol A (commercially available), and deuterated analogues of triethylene glycol dimethacrylate and urethane dimethacrylate (custom‐made). The optimized method was validated for linearity of the calibration curves and the associated correlation coefficient, lower limit of quantification, higher limit of quantification, and intra‐ and interassay accuracy and precision. Additionally, the developed liquid chromatography with tandem mass spectrometry method was applied to the analysis of leaching compounds from four resin‐based dental materials. The results indicated that this method is suitable for the analysis of different target compounds leaching from dental materials. This method might serve as a valuable basis for quick and accurate quantification of leached compounds from resin‐based dental materials in biological samples.