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Microwave‐assisted extraction versus Soxhlet extraction to determine triterpene acids in olive skins
Author(s) -
FernandezPastor Ignacio,
FernandezHernandez Antonia,
PerezCriado Sergio,
Rivas Francisco,
Martinez Antonio,
GarciaGranados Andres,
Parra Andres
Publication year - 2017
Publication title -
journal of separation science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.72
H-Index - 102
eISSN - 1615-9314
pISSN - 1615-9306
DOI - 10.1002/jssc.201601130
Subject(s) - chromatography , extraction (chemistry) , chemistry , triterpene , ethyl acetate , mass spectrometry , methanol , sample preparation , solvent , organic chemistry , medicine , alternative medicine , pathology
Microwave‐assisted extraction is compared with a more classical technique, Soxhlet extraction, to determine the content of triterpene acids in olive skins. The samples used in their original unmilled state and milled were extracted with ethyl acetate or methanol as solvents. The optimized operating conditions (e.g., amount and type of solvent, and time and temperature of extractions) to attain the better extraction yields have been established. For the identification and quantitation of the target compounds, an ultra high performance liquid chromatography with tandem mass spectrometry method was employed. The best results were achieved using the microwave‐assisted extraction technique, which was much faster than the Soxhlet extraction method, and showed higher efficiency in the extraction of the triterpenic acids (oleanolic and maslinic).