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Two‐dimensional solid‐phase extraction strategy for the selective enrichment of aminoglycosides in milk
Author(s) -
Shen Aijin,
Wei Jie,
Yan Jingyu,
Jin Gaowa,
Ding Junjie,
Yang Bingcheng,
Guo Zhimou,
Zhang Feifang,
Liang Xinmiao
Publication year - 2017
Publication title -
journal of separation science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.72
H-Index - 102
eISSN - 1615-9314
pISSN - 1615-9306
DOI - 10.1002/jssc.201601086
Subject(s) - solid phase extraction , chromatography , chemistry , elution , extraction (chemistry) , detection limit , mass spectrometry , dihydrostreptomycin , liquid chromatography–mass spectrometry , tandem mass spectrometry , european union , streptomycin , biochemistry , business , economic policy , antibiotics
An orthogonal two‐dimensional solid‐phase extraction strategy was established for the selective enrichment of three aminoglycosides including spectinomycin, streptomycin, and dihydrostreptomycin in milk. A reversed‐phase liquid chromatography material (C 18 ) and a weak cation‐exchange material (TGA) were integrated in a single solid‐phase extraction cartridge. The feasibility of two‐dimensional clean‐up procedure that experienced two‐step adsorption, two‐step rinsing, and two‐step elution was systematically investigated. Based on the orthogonality of reversed‐phase and weak cation‐exchange procedures, the two‐dimensional solid‐phase extraction strategy could minimize the interference from the hydrophobic matrix existing in traditional reversed‐phase solid‐phase extraction. In addition, high ionic strength in the extracts could be effectively removed before the second dimension of weak cation‐exchange solid‐phase extraction. Combined with liquid chromatography and tandem mass spectrometry, the optimized procedure was validated according to the European Union Commission directive 2002/657/EC. A good performance was achieved in terms of linearity, recovery, precision, decision limit, and detection capability in milk. Finally, the optimized two‐dimensional clean‐up procedure incorporated with liquid chromatography and tandem mass spectrometry was successfully applied to the rapid monitoring of aminoglycoside residues in milk.