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Evaluation of sample injection precision in respect to sensitivity in capillary electrophoresis using various injection modes
Author(s) -
Ciura Krzesimir,
Pawelec Aleksandra,
BuszewskaForajta Magdalena,
Markuszewski Michał Jan,
Nowakowska Joanna,
Prahl Adam,
Wielgomas Bartosz,
Dziomba Szymon
Publication year - 2017
Publication title -
journal of separation science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.72
H-Index - 102
eISSN - 1615-9314
pISSN - 1615-9306
DOI - 10.1002/jssc.201601027
Subject(s) - chromatography , capillary electrophoresis , analyte , detection limit , chemistry , reproducibility , analytical chemistry (journal) , capillary action , coefficient of variation , accuracy and precision , sensitivity (control systems) , repeatability , sample preparation , high performance liquid chromatography , materials science , mathematics , statistics , electronic engineering , engineering , composite material
A comparative study was conducted to assess the injection precision in capillary electrophoresis for cationic analytes (arecoline, codeine, papaverine). The precision was measured in respect to methods sensitivity in various injection modes in capillary electrophoresis: standard hydrodynamic injection (3.45 kPa for 6 s), large volume sample stacking (3.45 kPa for 40 s), and field‐amplified sample injection (10 kV for 65 s). All measurements were conducted for aqueous solutions of standards to minimize the errors linked to the sample preparation step. The methods were submitted to precision assessment at three concentration levels: at the limit of quantification, three‐fold and ten‐fold of limit of quantification. The results were compared to those from high‐performance liquid chromatography as a reference technique. The field‐amplified sample injection method was shown to provide greatest sensitivity (quantification limits down to 4 ng/mL for all three tested compounds) but the lowest precision. High‐performance liquid chromatography was established as the most reliable technique (coefficient of variation in all intraday experiments was below 1%). It was also shown that with a use of large volume sample injection technique, similar sensitivity as in high‐performance liquid chromatography can be easily reached.