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Comprehensive metabonomic analysis of heart tissue from isoproterenol‐induced myocardial infarction rat based on reversed‐phase and hydrophilic interaction chromatography coupled to mass spectrometry
Author(s) -
Sun Lei,
Liu Jianxun,
Sun Mingqian,
Lin Li,
Miao Lan,
Ge Zhengyan,
Yang Bin
Publication year - 2017
Publication title -
journal of separation science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.72
H-Index - 102
eISSN - 1615-9314
pISSN - 1615-9306
DOI - 10.1002/jssc.201601013
Subject(s) - taurine , chemistry , hypotaurine , chromatography , myocardial infarction , hydrophilic interaction chromatography , mass spectrometry , high performance liquid chromatography , biochemistry , amino acid , medicine
We aim to describe the metabonomic characteristics of myocardial infarction rats. High‐performance liquid chromatography coupled with quadrupole time‐of‐flight mass spectrometry was utilized to develop a metabonomic method of the heart homogenates of myocardial infarction rats. Hydrophilic interaction chromatography allows the analysis of high polar metabolites, providing complementary information to reversed‐phase liquid chromatography. We combined reversed phase and hydrophilic interaction chromatographic separations to analyze 18 samples, ten from myocardial infarction rat hearts and eight from normal rat hearts. A total of 16 potential biomarkers in rat heart tissue were screened out, primarily related to oxidative stress, nitric oxide damage, taurine, and hypotaurine metabolism and sphingolipid metabolism. This research showed that a comprehensive metabonomic study is a useful tool to reveal the underlying mechanism of myocardial infarction.