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Fast analysis of domoic acid using microchip electrophoresis with laser‐induced fluorescence detection
Author(s) -
Cheng Yongqiang,
Guo Cuilian,
Zhao Bin,
Yang Li
Publication year - 2017
Publication title -
journal of separation science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.72
H-Index - 102
eISSN - 1615-9314
pISSN - 1615-9306
DOI - 10.1002/jssc.201600982
Subject(s) - domoic acid , chromatography , detection limit , chemistry , laser induced fluorescence , analytical chemistry (journal) , fluorescence , reagent , biochemistry , physics , quantum mechanics , toxin
A fast and effective method was developed to detect domoic acid based upon microchip electrophoresis combined with laser‐induced fluorescence detection. Through study of the gated injection process on the cross channel of the microchip, the low‐voltage mode with relatively longer sample loading time was adopted to reduce the sample discrimination and improve the signal sensitivity. Fluorescein isothiocyanate was used as the derivatizing reagent for domoic acid. Under the optimized conditions, domoic acid was completely separated in 60 s with separation efficiency of 1.35 × 10 5 m −1 . The calibration curve was obtained in the range of 1.0 × 10 −9 to 1.0 × 10 −7 mol/L, and the detection limit reached 2.8 × 10 −10 mol/L. This developed method was successfully applied to analyze domoic acid in real samples.