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Simultaneous determination of trimethylamine and trimethylamine N ‐oxide in mouse plasma samples by hydrophilic interaction liquid chromatography coupled to tandem mass spectrometry
Author(s) -
Mi Si,
Zhao YuanYuan,
Jacobs René L.,
Curtis Jonathan M.
Publication year - 2017
Publication title -
journal of separation science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.72
H-Index - 102
eISSN - 1615-9314
pISSN - 1615-9306
DOI - 10.1002/jssc.201600926
Subject(s) - trimethylamine , trimethylamine n oxide , chromatography , chemistry , tandem mass spectrometry , mass spectrometry , liquid chromatography–mass spectrometry , tandem , hydrophilic interaction chromatography , quantitative analysis (chemistry) , high performance liquid chromatography , analytical chemistry (journal) , organic chemistry , materials science , composite material
A method was developed that applies hydrophilic interaction liquid chromatography with tandem mass spectrometry in the multiple reaction monitoring mode to separate and accurately quantify trimethylamine and trimethylamine N ‐oxide in a single chromatographic run. This was achieved by converting trimethylamine to ethyl betaine, which is less volatile and hence results in greatly improved quantitation. Ethyl betaine also gives a similar response to trimethylamine N ‐oxide using positive‐ion electrospray ionization mass spectrometry. It is readily separated from trimethylamine N ‐oxide by hydrophilic liquid chromatography in a 5 min run and with improved peak shape compared to underivatized trimethylamine. Validation of the method yielded a limit of detection (S/N ≥ 3) of 0.5 ng/mL for trimethylamine and 0.25 ng/mL for trimethylamine N ‐oxide. Method accuracies of 91.4–105.3% with precisions of 0.4–5.5% were obtained for standard mixtures over the range of 2.5–500 ng/mL. Recoveries measured for the extraction of trimethylamine and trimethylamine N ‐oxide spikes into mouse plasma were both >90%. The method, which simultaneously measures trimethylamine and trimethylamine N ‐oxide, was successfully applied to mouse plasma samples and could be adapted for use with other biological fluids.