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First separation of four aromatic acids and two analogues with similar structures and polarities from Clematis akebioides by high‐speed counter‐current chromatography
Author(s) -
Gong Chengcheng,
Chen Tao,
Chen Huifeng,
Zhang Shanshan,
Wang Xinzhu,
Wang Weiqing,
Sun Jing,
Li Yulin,
Liao Zhixin
Publication year - 2016
Publication title -
journal of separation science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.72
H-Index - 102
eISSN - 1615-9314
pISSN - 1615-9306
DOI - 10.1002/jssc.201600805
Subject(s) - countercurrent chromatography , chromatography , chemistry , acetonitrile , ethyl acetate , clematis , column chromatography , chloroform , silica gel , methanol , high performance liquid chromatography , solvent , fraction (chemistry) , organic chemistry , medicine , herb , medicinal herbs , traditional medicine
In this paper, we report an efficient method by high‐speed counter‐current chromatography for the first separation of four aromatic acids and two analogs with similar structures and polarities from Clematis akebioides . First, the ethyl acetate extract was treated by silica gel column chromatography to enrich the target compounds. And then the fraction with target compounds were purified by high‐speed counter‐counter chromatography using a two‐phase solvent system consisting of chloroform/acetonitrile/water (10:6:4, v/v). The results showed high‐speed counter‐current chromatography could be a powerful technology for the separation of compounds with similar structures and polarities. Besides, it was found acetonitrile could be a good methanol substitute when a chloroform/methanol/water system could not provide a good separation factor. This study provides a reference for the separation of compounds from Clematis akebioides .