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Simultaneous quantification of triterpenoid saponins in rat plasma by UHPLC–MS/MS and its application to a pharmacokinetic study after oral total saponin of Aralia elata leaves
Author(s) -
Sun Yichun,
Xue Juan,
Li Baimei,
Lin Xiaoting,
Wang Zhibin,
Jiang Hai,
Zhang Hongwei,
Wang Qiuhong,
Kuang Haixue
Publication year - 2016
Publication title -
journal of separation science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.72
H-Index - 102
eISSN - 1615-9314
pISSN - 1615-9306
DOI - 10.1002/jssc.201600801
Subject(s) - chemistry , chromatography , saponin , oleanolic acid , electrospray ionization , tandem mass spectrometry , selected reaction monitoring , analyte , protein precipitation , triterpenoid saponin , triterpene , high performance liquid chromatography , triple quadrupole mass spectrometer , mass spectrometry , medicine , alternative medicine , pathology
A rapid, sensitive, and reliable analytical ultra performance liquid chromatography with tandem mass spectrometry method was developed for the simultaneous determination of Aralia‐saponin IV, 3‐ O ‐β‐ d ‐glucopyranosyl‐(1→3)‐β‐ d ‐glucopyranosyl‐(1→3)‐β‐ d ‐glucopyranosyl oleanolic acid 28‐ O ‐β‐ d ‐glucopyranoside, Aralia‐saponin A and Aralia‐saponin B after the oral administration of total saponin of Aralia elata leaves in rat plasma. Plasma samples were pretreated by protein precipitation with methanol. The analysis was performed on an ACQUITY UPLC HSS T3 column. The detection was performed on a triple quadrupole tandem mass spectrometer in multiple reaction monitoring mode using an electrospray ionization source with negative ionization mode. Under the experimental conditions, the calibration curves of four analytes had good linearity values ( r > 0.991). The intra‐ and inter‐day precision values of the four analytes were ≤ 11.6%, and the accuracy was between –6.2 and 4.2%.The extraction recoveries of four triterpenoid saponins were in the range of 84.06–91.66% (RSD < 10.5%), and all values of the matrix effect were more than 90.30%. The developed analytical method was successfully applied to pharmacokinetic study on simultaneous determination of the four triterpenoid saponins in rat plasma after oral administration of total saponin of Aralia elata leaves, which helps guiding clinical usage of Aralia elata leaves.