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Overview of online two‐dimensional liquid chromatography based on cell membrane chromatography for screening target components from traditional Chinese medicines
Author(s) -
Muhammad Saqib,
Han Shengli,
Xie Xiaoyu,
Wang Sicen,
Aziz Muhammad Majid
Publication year - 2017
Publication title -
journal of separation science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.72
H-Index - 102
eISSN - 1615-9314
pISSN - 1615-9306
DOI - 10.1002/jssc.201600773
Subject(s) - chromatography , chemistry , column chromatography , chromatography column , countercurrent chromatography , membrane , high performance liquid chromatography , biochemistry
Cell membrane chromatography is a simple, specific, and time‐saving technique for studying drug–receptor interactions, screening of active components from complex mixtures, and quality control of traditional Chinese medicines. However, the short column life, low sensitivity, low column efficiency (so cannot resolve satisfactorily mixture of compounds), low peak capacity, and inefficient in structure identification were bottleneck in its application. Combinations of cell membrane chromatography with multidimensional chromatography such as two‐dimensional liquid chromatography and high sensitivity detectors like mass have significantly reduced many of the above‐mentioned shortcomings. This paper provides an overview of the current advances in online two‐dimensional‐based cell membrane chromatography for screening target components from traditional Chinese medicines with particular emphasis on the instrumentation, preparation of cell membrane stationary phase, advantages, and disadvantages compared to alternative approaches. The last section of the review summarizes the applications of the online two‐dimensional high‐performance liquid chromatography based cell membrane chromatography reported since its emergence to date (2010–June 2016).