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Determination of sophoraflavanone G and kurarinone in rat plasma by UHPLC–MS/MS and its application to a pharmacokinetic study
Author(s) -
Yang Zhixin,
Zhang Wenjun,
Li Xia,
Shan Baisong,
Liu Jiajia,
Deng Weizhe
Publication year - 2016
Publication title -
journal of separation science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.72
H-Index - 102
eISSN - 1615-9314
pISSN - 1615-9306
DOI - 10.1002/jssc.201600681
Subject(s) - chromatography , chemistry , sophora flavescens , triple quadrupole mass spectrometer , electrospray ionization , pharmacokinetics , rutin , selected reaction monitoring , mass spectrometry , bioavailability , ethyl acetate , analyte , tandem mass spectrometry , pharmacology , medicine , matrine , biochemistry , antioxidant
This study aimed to develop and validate a simple and sensitive ultra high performance liquid chromatography tandem mass spectrometry method for the simultaneous determination of sophoraflavanone G and kurarinone in rat plasma by using rutin as the internal standard. Then, the developed method was applied to investigate the pharmacokinetics of sophoraflavanone G and kurarinone in rats after dosing the flavonoid extract from Sophora flavescens . Plasma samples were processed using a liquid–liquid extraction procedure with ethyl acetate. The analysis was performed on a triple quadrupole tandem mass spectrometer by multiple reaction monitoring with an electrospray ionization source in negative ionization mode. Quantitative ion transitions of m/z 423.2→161.2, 437.2→161.1, and 609.3→300.3 were monitored for sophoraflavanone G, kurarinone, and rutin, respectively. The calibration curves of the two analytes exhibited good linearity ( r 2 >0.9923) over the range of 0.1–200 ng/mL for sophoraflavanone G and 0.1–1000 ng/mL for kurarinone. Relative standard deviations were less than 13.2% for the intra‐ and inter‐day precisions and no more than 12.6% for the recovery, showing good precision and satisfactory accuracy of the developed method. The validated method was successfully applied to the pharmacokinetic study of sophoraflavanone G and kurarinone after a single intravenous (25 mg/kg) and oral (500 mg/kg) administration of the flavonoid extract from S. flavescens , and the absolute bioavailability for sophoraflavanone G and kurarinone was about 36 and 17%, respectively.