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Fast determination of anthocyanins and free pelargonidin in fruits, fruit juices, and fruit wines by high‐performance liquid chromatography using a core–shell column
Author(s) -
Yıldırım Sercan,
Kadıoğlu Asım,
Sağlam Aykut,
Yaşar Ahmet,
Sellitepe Hasan Erdinç
Publication year - 2016
Publication title -
journal of separation science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.72
H-Index - 102
eISSN - 1615-9314
pISSN - 1615-9306
DOI - 10.1002/jssc.201600661
Subject(s) - chemistry , chromatography , detection limit , formic acid , high performance liquid chromatography , acetonitrile , anthocyanin , pelargonidin , analyte , cyanidin , food science
Anthocyanins are water‐soluble pigments that are liable for colors ranging from red to blue of most fruits, vegetables, and flowers. A novel and fast method was developed for the determination of five anthocyanins and free pelargonidin by high‐performance liquid chromatography coupled to photodiode array detection. A 10% formic acid and acetonitrile mixture was employed as mobile phase in the gradient elution mode. Mobile phase composition, column temperature, flow rate, injection volume, and column conditioning time were optimized by employing a stepwise strategy. Using a C 18 core–shell column (100 × 4.6 mm, 2.7 μm), the separation of six analytes was accomplished in less than 9.5 min with a run‐to‐run analysis time of 19 min. The developed method was validated with respect to linearity ( r > 0.9999), limit of detection, limit of quantification, intra‐/interday precision (<2%), accuracy (98.6–104.4%), and specificity. Afterwards, the method was applied to the determination of anthocyanins present in 15 different samples including fruits, fruit juices, and fruit wines.