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Gadolinium oxide: Exclusive selectivity and sensitivity in the enrichment of phosphorylated biomolecules
Author(s) -
Jabeen Fahmida,
NajamulHaq Muhammad,
Ashiq Muhammad Naeem,
Rainer Matthias,
Huck Christian W.,
Bonn Guenther K.
Publication year - 2016
Publication title -
journal of separation science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.72
H-Index - 102
eISSN - 1615-9314
pISSN - 1615-9306
DOI - 10.1002/jssc.201600651
Subject(s) - phosphopeptide , chemistry , chromatography , selectivity , gadolinium , bovine serum albumin , phosphoproteomics , biomolecule , electrospray , detection limit , mass spectrometry , nanoparticle , peptide , biochemistry , phosphorylation , nanotechnology , protein phosphorylation , organic chemistry , materials science , protein kinase a , catalysis
Selectivity and sensitivity define the dynamic applicability of separation and enrichment techniques. Owing to proteome complexity, numbers of separation media have been introduced in phosphoproteomics. Complex samples are pretreated to make the low‐abundance molecules detectable by mass spectrometry. Gadolinium oxide nanoparticles, offering mono‐ and bi‐dentate interactions, are optimized to capture the phosphopeptides. Selectivity of 1:11 000 is achieved for digested β‐casein phosphopeptides in bovine serum albumin digest background using gadolinium oxide nanoparticles. The limit of detection goes down to 1 attomole. With the optimized sample preparation protocol, gadolinium oxide nanoparticles enrich phosphopeptides of κ‐casein (Ser 148 and Ser 170 ) from digested milk sample, fibrinogen alpha chain phosphopeptide (Ser 609 ) along with four hydrolytic products of Ser 22 ‐modified phosphopeptides from serum.