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Preconcentration and determination of ceftazidime in real samples using dispersive liquid–liquid microextraction and high‐performance liquid chromatography with the aid of experimental design
Author(s) -
Razmi Rasoul,
Shahpari Behrouz,
Pourbasheer Eslam,
Boustanifar Mohammad Hasan,
Azari Zhila,
Ebadi Amin
Publication year - 2016
Publication title -
journal of separation science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.72
H-Index - 102
eISSN - 1615-9314
pISSN - 1615-9306
DOI - 10.1002/jssc.201600639
Subject(s) - chromatography , high performance liquid chromatography , liquid liquid , ceftazidime , chemistry , materials science , analytical chemistry (journal) , biology , bacteria , pseudomonas aeruginosa , genetics
A rapid and simple method for the extraction and preconcentration of ceftazidime in aqueous samples has been developed using dispersive liquid–liquid microextraction followed by high‐performance liquid chromatography analysis. The extraction parameters, such as the volume of extraction solvent and disperser solvent, salt effect, sample volume, centrifuge rate, centrifuge time, extraction time, and temperature in the dispersive liquid–liquid microextraction process, were studied and optimized with the experimental design methods. Firstly, for the preliminary screening of the parameters the taguchi design was used and then, the fractional factorial design was used for significant factors optimization. At the optimum conditions, the calibration curves for ceftazidime indicated good linearity over the range of 0.001–10 μg/mL with correlation coefficients higher than the 0.98, and the limits of detection were 0.13 and 0.17 ng/mL, for water and urine samples, respectively. The proposed method successfully employed to determine ceftazidime in water and urine samples and good agreement between the experimental data and predictive values has been achieved.