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High‐throughput ultra high performance liquid chromatography coupled to quadrupole time‐of‐flight mass spectrometry method for the rapid analysis and characterization of multiple constituents of Radix Polygalae
Author(s) -
Liu Chang,
Zhang Aihua,
Yan Guangli,
Shi Hui,
Sun Hui,
Han Ying,
Zhou Ying,
Wang Xijun
Publication year - 2017
Publication title -
journal of separation science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.72
H-Index - 102
eISSN - 1615-9314
pISSN - 1615-9306
DOI - 10.1002/jssc.201600624
Subject(s) - quadrupole time of flight , radix (gastropod) , mass spectrometry , chromatography , chemistry , in vivo , time of flight mass spectrometry , chemical constituents , high performance liquid chromatography , tandem mass spectrometry , biology , botany , ion , microbiology and biotechnology , organic chemistry , ionization
Radix Polygalae, the dried roots of Polygala tenuifolia and P. sibirica , is one of the most well‐known traditional Chinese medicinal plants. It is an important medicinal plant that has been used as a sedative and to improve memory for a number of years in most of Asia. However, the in vivo constituents of the multiple constituents from Radix Polygalae remain unknown. In the current study, ultra high performance liquid chromatography coupled to quadrupole time‐of‐flight mass spectrometry and the MarkerLynx TM software combined with multiple data processing approach were used to study the constituents in vitro and in vivo. A rapid and efficient method for the characterization of multiple constituents in the herbal medicine Radix Polygalae by ultra high performance liquid chromatography coupled to quadrupole time‐of‐flight mass spectrometry is described. In total, 35 compounds in the Radix Polygalae and 13 compounds absorbed into blood were characterized. Of the 35 compounds in vitro, ten were reported for first time. In the 13 compounds in vivo, six were prototype components and seven were metabolites were also elucidated for first time. This work narrowed the range of screening the potentially bioactive components and provided a basis for the quality control and mechanism of action.