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Multiclass method for the quantification of 92 veterinary antimicrobial drugs in livestock excreta, wastewater, and surface water by liquid chromatography with tandem mass spectrometry
Author(s) -
Gao Jinfang,
Cui Yonghui,
Tao Yanfei,
Huang Lingli,
Peng Dapeng,
Xie Shuyu,
Wang Xu,
Liu Zhenli,
Chen Dongmei,
Yuan Zonghui
Publication year - 2016
Publication title -
journal of separation science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.72
H-Index - 102
eISSN - 1615-9314
pISSN - 1615-9306
DOI - 10.1002/jssc.201600531
Subject(s) - chromatography , chemistry , solid phase extraction , tandem mass spectrometry , antimicrobial , extraction (chemistry) , liquid chromatography–mass spectrometry , mass spectrometry , urine , veterinary drug , detection limit , veterinary drugs , electrospray ionization , veterinary medicine , medicine , biochemistry , organic chemistry
A simple multiresidue method was developed for detecting and quantifying 92 veterinary antimicrobial drugs from eight classes (β‐lactams, quinolones, sulfonamides, tetracyclines, lincomycins, macrolides, chloramphenicols, and pleuromutilin) in livestock excreta and water by liquid chromatography with tandem mass spectrometry. The feces samples were extracted by ultrasound‐assisted extraction with a mixture of acetonitrile/water (80:20, v/v) and edetate disodium, followed by a cleanup using solid‐phase extraction with an amino cartridge. Water samples were purified with hydrophilic–lipophilic balance solid‐phase extraction column. Urine samples were extracted with acetonitrile and edetate disodium. Detection of veterinary antimicrobial drugs was achieved by liquid chromatography with tandem mass spectrometry using both positive and negative electrospray ionization mode. The recovery values of veterinary antimicrobial drugs in feces, urine, and water samples were 75–99, 85–110, and 85–101% and associated relative standard deviations were less than 15, 10, and 8%, respectively. The limits of quantification in feces, urine, and water samples were 0.5–1, 0.5–1, and 0.01–0.05 μg/L, respectively. This method was applied to determine real samples obtained from local farms and provides reliable quantification and identification results of 92 veterinary antimicrobial drugs in livestock excreta and water.