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Simultaneous determination of notoginsenoside R1 and ginsenoside Re in rat plasma by ultra high performance liquid chromatography with tandem mass spectrometry and its application to a pharmacokinetic study
Author(s) -
Dai Guoliang,
Jiang Zhitao,
Zhu Lijing,
Zhang Qian,
Zong Yang,
Liu Shijia,
Li Changyin,
Ju Wenzheng
Publication year - 2016
Publication title -
journal of separation science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.72
H-Index - 102
eISSN - 1615-9314
pISSN - 1615-9306
DOI - 10.1002/jssc.201600522
Subject(s) - chromatography , chemistry , pharmacokinetics , tandem mass spectrometry , mass spectrometry , high performance liquid chromatography , tandem , liquid chromatography–mass spectrometry , ginsenoside rg1 , ginsenoside , pharmacology , ginseng , medicine , materials science , alternative medicine , pathology , composite material
A rapid and high sensitive ultra high performance liquid chromatography with tandem mass spectrometry method for the simultaneous determination of notoginsenoside R1 and ginsenoside Re in rat plasma was developed. The analytes and internal standard, digoxin, were extracted from rat plasma via protein precipitation with methanol and separated on an Phenomenex Gemini C 18 column within 2 min. Quantitation was performed on a triple quadrupole mass spectrometer employing electrospray ionization technique, operating in multiple reaction monitoring and positive ion mode. The precursor to product ion transitions monitored for notoginsenoside R1, ginsenoside Re, and internal standard were m/z 955.5→775.5, 969.6→789.1, and 803.6→283.1, respectively. The assay was validated with linear range of 1.9–380 ng/mL for notoginsenoside R1 and 0.5–100 ng/mL for ginsenoside Re. The intra‐ and interday precisions (RSD%) were within 8.96% for each analyte. The absolute recoveries were greater than 93% for R1 and 96% for Re. Each analyte was stable during all sample storage, preparation, and analytic procedures. The method was successfully applied to a pharmacokinetic study of Xuesaitong dispersible tablets in eight rats.