Premium
Quick method for separating target compounds from the bark of Maqian ( Zanthoxylum myriacanthum var. pubescens ) by high‐performance countercurrent chromatography
Author(s) -
Fan Qingfei,
Zhang Huanli,
Hu Huabin,
Xu Youkai,
Song Qishi
Publication year - 2016
Publication title -
journal of separation science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.72
H-Index - 102
eISSN - 1615-9314
pISSN - 1615-9306
DOI - 10.1002/jssc.201600521
Subject(s) - countercurrent chromatography , chromatography , chemistry , ethyl acetate , solvent , countercurrent exchange , thin layer chromatography , column chromatography , hexane , organic chemistry , physics , thermodynamics
Choosing a suitable solvent system for a countercurrent chromatography separation presents a challenge for many researchers. In this study, we introduce a quick method of separating a target compound from the bark of Zanthoxylum myriacanthum var. pubescens by countercurrent chromatography. This method relies on the thin‐layer chromatography based generally useful estimation of solvent systems. This paper will present how to quickly choose a suitable solvent system with a thin‐layer chromatography based generally useful estimation of solvent systems working chart. O ‐Methyltembamide ( 1 ) was enriched by countercurrent chromatography using n ‐hexane/ethyl acetate/methanol/water (6:4:6:4) as the solvent system. Further purification was achieved by high‐performance liquid chromatography with purities of 98.2% from Z. myriacanthum var. pubescens bark .