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Simultaneous speciation analysis of inorganic nitrogen with the use of ion chromatography in highly salinated environmental samples
Author(s) -
Kurzyca Iwona,
Niedzielski Przemyslaw,
Frankowski Marcin
Publication year - 2016
Publication title -
journal of separation science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.72
H-Index - 102
eISSN - 1615-9314
pISSN - 1615-9306
DOI - 10.1002/jssc.201600514
Subject(s) - chemistry , reagent , derivatization , ion chromatography , chromatography , nitrogen , detection limit , inorganic ions , mass spectrometry , genetic algorithm , environmental chemistry , ion , organic chemistry , evolutionary biology , biology
We present the development of a method for the simultaneous determination of inorganic nitrogen species in oxidized (NO 2 – , NO 3 – ) and reduced (NH 4 + ) forms using ion chromatography with diode‐array detection (205, 208, and 425 nm, respectively). The oxidized forms were determined directly after the separation in the anion exchanger, while the reduced form was determined in the column hold‐up time after derivatization with the Nessler reagent. The use of an appropriate modifier (Seignette reagent) and mobile phase (NaCl) enabled the determination of inorganic nitrogen species in highly salinated environmental samples (water, sediments). Moreover, low detection limits were obtained of 0.04 mg/L for NH 4 + and 0.006 and 0.005 mg/L for NO 2 – and NO 3 – , respectively. The analysis of environmental samples indicated NH 4 + contents of up to 1161 ± 47 mg/kg and NO 3 – of up to 148 ± 6 mg/kg for sediment samples, as well as the NH 4 + concentrations of up to 0.98 ± 0.10 mg/L, NO 2 – of up to 24 ± 1 mg/L and NO 3 – of up to 20 ± 1 mg/L for water samples.