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Multivariate analysis of the volatile components in tobacco based on infrared‐assisted extraction coupled to headspace solid‐phase microextraction and gas chromatography‐mass spectrometry
Author(s) -
Yang Yanqin,
Pan Yuanjiang,
Zhou Guojun,
Chu Guohai,
Jiang Jian,
Yuan Kailong,
Xia Qian,
Cheng Changhe
Publication year - 2016
Publication title -
journal of separation science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.72
H-Index - 102
eISSN - 1615-9314
pISSN - 1615-9306
DOI - 10.1002/jssc.201600503
Subject(s) - solid phase microextraction , chemometrics , chromatography , extraction (chemistry) , mass spectrometry , sample preparation , gas chromatography–mass spectrometry , gas chromatography , chemistry , principal component analysis , analytical chemistry (journal) , computer science , artificial intelligence
A novel infrared‐assisted extraction coupled to headspace solid‐phase microextraction followed by gas chromatography with mass spectrometry method has been developed for the rapid determination of the volatile components in tobacco. The optimal extraction conditions for maximizing the extraction efficiency were as follows: 65 μm polydimethylsiloxane‐divinylbenzene fiber, extraction time of 20 min, infrared power of 175 W, and distance between the infrared lamp and the headspace vial of 2 cm. Under the optimum conditions, 50 components were found to exist in all ten tobacco samples from different geographical origins. Compared with conventional water‐bath heating and nonheating extraction methods, the extraction efficiency of infrared‐assisted extraction was greatly improved. Furthermore, multivariate analysis including principal component analysis, hierarchical cluster analysis, and similarity analysis were performed to evaluate the chemical information of these samples and divided them into three classifications, including rich, moderate, and fresh flavors. The above‐mentioned classification results were consistent with the sensory evaluation, which was pivotal and meaningful for tobacco discrimination. As a simple, fast, cost‐effective, and highly efficient method, the infrared‐assisted extraction coupled to headspace solid‐phase microextraction technique is powerful and promising for distinguishing the geographical origins of the tobacco samples coupled to suitable chemometrics.