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Separation of three anthraquinone glycosides including two isomers by preparative high‐performance liquid chromatography and high‐speed countercurrent chromatography from Rheum tanguticum Maxim. ex Balf
Author(s) -
Chen Tao,
Li Hongmei,
Zou Denglang,
Liu Yongling,
Chen Chen,
Zhou Guoying,
Li Yulin
Publication year - 2016
Publication title -
journal of separation science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.72
H-Index - 102
eISSN - 1615-9314
pISSN - 1615-9306
DOI - 10.1002/jssc.201600487
Subject(s) - chemistry , countercurrent chromatography , glycoside , anthraquinone , chromatography , glucoside , high performance liquid chromatography , organic chemistry , medicine , alternative medicine , pathology
Anthraquinone glycosides, such as chrysophanol 1‐ O ‐β ‐d‐ glucoside, chrysophanol 8‐ O ‐β ‐d‐ glucoside, and physion 8‐ O ‐β ‐d‐ glucoside, are the accepted important active components of Rheum tanguticum Maxim. ex Balf. due to their pharmacological properties: antifungal, antimicrobial, cytotoxic, and antioxidant activities. However, an effective method for the separation of the above‐mentioned anthraquinone glycosides from this herb is not currently available. Especially, greater difficulty existed in the separation of the two isomers chrysophanol 1‐ O ‐β ‐d‐ glucoside and chrysophanol 8‐ O ‐β ‐d‐ glucoside. This study demonstrated an efficient strategy based on preparative high‐performance liquid chromatography and high‐speed countercurrent chromatography for the separation of the above‐mentioned anthraquinone glycosides from Rheum tanguticum Maxim.ex Balf.