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Novel method for the rapid and specific extraction of multiple β 2 ‐agonist residues in food by tailor‐made Monolith‐MIPs extraction disks and detection by gas chromatography with mass spectrometry
Author(s) -
Liu Haibo,
Gan Ning,
Chen Yinji,
Ding Qingqing,
Huang Jie,
Lin Saichai,
Cao Yuting,
Li Tianhua
Publication year - 2016
Publication title -
journal of separation science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.72
H-Index - 102
eISSN - 1615-9314
pISSN - 1615-9306
DOI - 10.1002/jssc.201600479
Subject(s) - chromatography , clenbuterol , monolith , chemistry , mass spectrometry , extraction (chemistry) , elution , desorption , solid phase extraction , detection limit , molecularly imprinted polymer , derivatization , sample preparation , gas chromatography , adsorption , selectivity , organic chemistry , catalysis
A quick and specific pretreatment method based on a series of extraction clean‐up disks, consisting of molecularly imprinted polymer monoliths and C 18 adsorbent, was developed for the specific enrichment of salbutamol and clenbuterol residues in food. The molecularly imprinted monolithic polymer disk was synthesized using salbutamol as a template through a one‐step synthesis process. It can simultaneously and specifically recognize salbutamol and clenbuterol. The monolithic polymer disk and series of C 18 disks were assembled with a syringe to form a set of tailor‐made devices for the extraction of target molecules. In a single run, salbutamol and clenbuterol can be specifically extracted, cleaned, and eluted by methanol/acetic acid/H 2 O. The target molecules, after a silylation derivatization reaction were detected by gas chromatography‐mass spectrometry. The parameters including solvent desorption, sample pH, and the cycles of reloading were investigated and discussed. Under the optimized extraction and clean‐up conditions, the limits of detection and quantitation were determined as 0.018–0.022 and 0.042–0.049 ng/g for salbutamol and clenbuterol, respectively. The assay described was convenient, rapid, and specific; thereby potentially efficient in the high‐throughput analysis of β 2 ‐agonists residues in real food samples.