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Simultaneous determination of six synthetic phenolic antioxidants in edible oils using dispersive liquid–liquid microextraction followed by high‐performance liquid chromatography with diode array detection
Author(s) -
Xu Shuangjiao,
Liu Liangliang,
Wang Yanqin,
Zhou Dayun,
Kuang Meng,
Fang Dan,
Yang Weihua,
Wei Shoujun,
Xiao Aiping,
Ma Lei
Publication year - 2016
Publication title -
journal of separation science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.72
H-Index - 102
eISSN - 1615-9314
pISSN - 1615-9306
DOI - 10.1002/jssc.201600434
Subject(s) - chromatography , chemistry , extraction (chemistry) , high performance liquid chromatography , detection limit , sample preparation , chromatography detector , solvent , organic chemistry
A simple, rapid, organic‐solvent‐ and sample‐saving pretreatment technique, called dispersive liquid–liquid microextraction, was developed for the determination of six synthetic phenolic antioxidants from edible oils before high‐performance liquid chromatography with diode array detection. The entire procedure was composed of a two‐step microextraction and a centrifugal process and could be finished in about 5 min, only consuming only 25 mg of sample and 1 mL of the organic solvent for each extraction. The influences of several important parameters on the microextraction efficiency were thoroughly investigated. Recovery assays for oil samples were spiked at three concentration levels, 50, 100 and 200 mg/kg, and provided recoveries in the 86.3–102.5% range with a relative standard deviation below 3.5%. The intra‐day and inter‐day precisions for the analysis were less than 3.8%. The proposed method was successfully applied for the determination of synthetic phenolic antioxidants in different oil samples, and satisfactory results were obtained. Thus, the developed method represents a viable alternative for the quality control of synthetic phenolic antioxidant concentrations in edible oils.