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Extraction and isolation of lithospermic acid B from Salvia miltiorrhiza Bunge using aqueous two‐phase extraction followed by high‐performance liquid chromatography
Author(s) -
Guo Yong Xue,
Shi Chang Zhi,
Zhang Lei,
Lv Lin,
Zhang Yue Yong
Publication year - 2016
Publication title -
journal of separation science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.72
H-Index - 102
eISSN - 1615-9314
pISSN - 1615-9306
DOI - 10.1002/jssc.201600368
Subject(s) - salvia miltiorrhiza , chromatography , extraction (chemistry) , aqueous solution , chemistry , yield (engineering) , countercurrent chromatography , solvent , partition coefficient , aqueous two phase system , high performance liquid chromatography , materials science , organic chemistry , medicine , alternative medicine , pathology , traditional chinese medicine , metallurgy
A rapid and effective method integrating separation and purification of lithospermic acid B from Salvia miltiorrhiza Bunge was developed by combining an aqueous two‐phase system extraction with preparative chromatography. An aqueous two‐phase system of n ‐butyl alcohol/KH 2 PO 4 was chosen from seven systems. The influence of parameters including concentration of KH 2 PO 4 , n ‐butyl alcohol concentration, pH, and the ratio of an aqueous two‐phase system to crude extract were investigated using a single factor design. Response surface methodology was subsequently used to find the optimal compositions of an aqueous two‐phase system. Keeping a solvent‐to‐solid ratio of 10, the final optimized composition of an aqueous two‐phase system was 39.1% w/w n ‐butyl alcohol and 22.6% w/w KH 2 PO 4 . Under these conditions a recovery yield of 99.8% and a high partition coefficient of 310.4 were obtained. In a pilot‐scale experiment using optimized conditions, 18.79 g of lithospermic acid B with a purity of 70.5% and in a yield of 99.8% was separated from 0.5 kg of crude extract. Subsequently, 9.94 g lithospermic acid B with a purity of 99.3% and recovery yield of 70.3% was obtained with a preparative chromatographic process, and the two‐step total recovery was 70.1%.