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Multiple‐injection high‐throughput gas chromatography analysis
Author(s) -
Schafer Wes,
Wang Heather,
Welch Christopher J.
Publication year - 2016
Publication title -
journal of separation science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.72
H-Index - 102
eISSN - 1615-9314
pISSN - 1615-9306
DOI - 10.1002/jssc.201600332
Subject(s) - throughput , analyte , chromatography , mass spectrometry , chemistry , gas chromatography , analytical chemistry (journal) , computer science , telecommunications , wireless
Multiple‐injection techniques have been shown to be a simple way to perform high‐throughput analysis where the entire experiment resides in a single chromatogram, simplifying the data analysis and interpretation. In this study, multiple‐injection techniques are applied to gas chromatography with flame ionization detection and mass detection to significantly increase sample throughput. The unique issues of implementing a traditional “Fast” injection mode of multiple‐injection techniques with gas chromatography and mass spectrometry are discussed. Stacked injections are also discussed as means to increase the throughput of longer methods where mass detection is unable to distinguish between analytes of the same mass and longer retentions are required to resolve components of interest. Multiple‐injection techniques are shown to increase instrument throughput by up to 70% and to simplify data analysis, allowing hits in multiple parallel experiments to be identified easily.