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Simultaneous extraction and quantification of albendazole and triclabendazole using vortex‐assisted hollow‐fiber liquid‐phase microextraction combined with high‐performance liquid chromatography
Author(s) -
Asadi Mohammad,
Haji Shabani Ali Mohammad,
Dadfarnia Shayessteh
Publication year - 2016
Publication title -
journal of separation science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.72
H-Index - 102
eISSN - 1615-9314
pISSN - 1615-9306
DOI - 10.1002/jssc.201600286
Subject(s) - chromatography , albendazole , extraction (chemistry) , triclabendazole , chemistry , detection limit , calibration curve , high performance liquid chromatography , sample preparation , derivatization , solid phase extraction , medicine , surgery , helminths , fasciola hepatica , immunology
A novel, simple, and rapid vortex‐assisted hollow‐fiber liquid‐phase microextraction method was developed for the simultaneous extraction of albendazole and triclabendazole from various matrices before their determination by high‐performance liquid chromatography with fluorescence detection. Several factors influencing the microextraction efficiency including sample pH, nature and volume of extraction solvent, ionic strength, vortex time, and sample volume were investigated and optimized. Under the optimal conditions, the limits of detection were 0.08 and 0.12 μg/L for albendazole and triclabendazole, respectively. The calibration curves were linear in the concentration ranges of 0.3–50.0 and 0.4–50.0 μg/L with the coefficients of determination of 0.9999 and 0.9995 for albendazole and triclabendazole, respectively. The interday and intraday relative standard deviations for albendazole and triclabendazole at three concentration levels (1.0, 10.0, and 30.0 μg/L) were in the range of 6.0–11.0 and 5.0–7.9%, respectively. The developed method was successfully applied to determine albendazole and triclabendazole in water, milk, honey, and urine samples.