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Tartaric acid induced conversion of protopanaxadiol to ginsenosides Rg 3 and Rg 5 and their in situ recoveries by integrated expanded bed adsorption chromatography
Author(s) -
Huang Dan,
Li Yang,
Zhang Min,
Ruan Shengli,
Zhang Hongyang,
Wang Yuerong,
Hu Ping
Publication year - 2016
Publication title -
journal of separation science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.72
H-Index - 102
eISSN - 1615-9314
pISSN - 1615-9306
DOI - 10.1002/jssc.201600269
Subject(s) - protopanaxadiol , ginseng , ginsenoside , chemistry , chromatography , tartaric acid , extraction (chemistry) , adsorption , dammarane , sapogenin , herb , citric acid , medicinal herbs , triterpene , traditional medicine , organic chemistry , medicine , alternative medicine , pathology
Panax ginseng has been applied in traditional Chinese medicine for over 2000 years. It is still one of the most popular herbs in recent decades. The prescribed ginseng‐containing medicines consist of protopanaxadiol and protopanaxatriol ginsenosides, which are the major constituents of the herb. Minor ginsenosides at low levels in the herb, such as Rg 3 and Rg 5 , have attracted more rising attention than the major ones. The existing approaches to prepare Rg 3 and Rg 5 usually rely on either steamed red ginseng as the source or chemical/enzymatic conversion of protopanaxadiol to the targets. It is still highly desirable to effectively achieve such minor components. In this paper, a method integrated extraction of protopanaxadiol and conversion of it to Rg 3 and Rg 5 has been proposed. Protopanaxadiol was extracted and simultaneously converted to Rg 3 and Rg 5 by d , l ‐tartaric acid. The targets were absorbed by resins on expanded bed adsorption chromatography and were then separated from other ginsenosides in different stages. Compared with conventional methods, the developed process has advantages in shortening time consumption and improving the conversion ratio of protopanaxadiol, which is promising in directly achieving Rg 3 and Rg 5 from P . ginseng.