Premium
Simultaneous separation and determination of six arsenic species in rice by anion‐exchange chromatography with inductively coupled plasma mass spectrometry
Author(s) -
Ma Li,
Yang Zhaoguang,
Tang Jie,
Wang Lin
Publication year - 2016
Publication title -
journal of separation science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.72
H-Index - 102
eISSN - 1615-9314
pISSN - 1615-9306
DOI - 10.1002/jssc.201600216
Subject(s) - arsenobetaine , chemistry , arsenate , inductively coupled plasma mass spectrometry , chromatography , arsenic , arsenite , mass spectrometry , elution , ion chromatography , ion exchange , analytical chemistry (journal) , ion , organic chemistry
The simultaneous separation and determination of arsenite As(III), arsenate As(V), monomethylarsonic acid (MMA), dimethylarsinic acid (DMA), arsenobetaine (AsB), and arsenocholine (AsC) in rice samples have been carried out in one single anion‐exchange column run by high‐performance liquid chromatography with inductively coupled plasma mass spectrometry. To estimate the effect of variables on arsenic (As) speciation, the chromatographic conditions including type of competing anion, ionic strength, pH of elution buffer, and flow rate of mobile phase have been investigated by a univariate approach. Under the optimum chromatographic conditions, baseline separation of six As species has been achieved within 10 min by gradient elution program using 4 mM NH 4 HCO 3 at pH 8.6 as mobile phase A and 4 mM NH 4 HCO 3 , 40 mM NH 4 NO 3 at pH 8.6 as mobile phase B. The method detection limits for As(III), As(V), MMA, DMA, AsB, and AsC were 0.4, 0.9, 0.2, 0.4, 0.5, and 0.3 μg/kg, respectively. The proposed method has been applied to separation and quantification of As species in real rice samples collected from Hunan Province, China. The main As species detected in all samples were As(III), As(V) and DMA, with inorganic As accounting for over 80% of total As in these samples.