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Development of an ultrasound‐assisted emulsification microextraction method for the determination of chlorpyrifos and organochlorine pesticide residues in honey samples using gas chromatography with mass spectrometry
Author(s) -
Mousavi MirMichael,
Arefhosseini Seyedrafie,
Alizadeh Nabili Ali Akbar,
Mahmoudpour Mansour,
Nemati Mahboob
Publication year - 2016
Publication title -
journal of separation science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.72
H-Index - 102
eISSN - 1615-9314
pISSN - 1615-9306
DOI - 10.1002/jssc.201600197
Subject(s) - chromatography , chemistry , extraction (chemistry) , mass spectrometry , pesticide residue , gas chromatography , gas chromatography–mass spectrometry , selected ion monitoring , pesticide , solvent , detection limit , organic chemistry , agronomy , biology
A simple, rapid, and efficient ultrasound‐assisted emulsification microextraction method followed by gas chromatography mass spectrometry in selected ion monitoring mode was developed for the determination of organochlorine pesticides in honey samples. The type and volume of organic extraction solvent, pH, effect of added salt content, and centrifuging time and speed were investigated. Under the optimum extraction conditions, 30 μL of 1, 2‐dibromoethane (extraction solvent) was immersed into an ultrasonic bath for 1 min at 40°C. The limits of detection and quantification for all target pesticides were 0.003–0.06 and 0.01–0.2 ng/g , respectively. The extraction recovery was 91–100% and the enrichment factors were 168–192. The relative standard deviation for the method was <6% for intraday ( n = 6) and <8% for interday precision ( n = 4). The proposed method was successfully applied for the analysis of organochlorine pesticides in honey samples.