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Preparation of magnetic glycoprotein‐imprinted nanoparticles with dendritic polyethyleneimine as a monomer for the specific recognition of ovalbumin from egg white
Author(s) -
Zhang Lili,
Tang Yuhai,
Hao Yi,
He Gaiyan,
Zhang Bianbian,
Gao Ruixia,
Zhang Min
Publication year - 2016
Publication title -
journal of separation science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.72
H-Index - 102
eISSN - 1615-9314
pISSN - 1615-9306
DOI - 10.1002/jssc.201600112
Subject(s) - ovalbumin , egg white , polymer , molecular imprinting , biocompatibility , molecularly imprinted polymer , nanoparticle , chemistry , monomer , imprinting (psychology) , superparamagnetism , adsorption , magnetic nanoparticles , chemical engineering , materials science , chromatography , biophysics , nanotechnology , selectivity , biochemistry , organic chemistry , catalysis , biology , immune system , magnetization , physics , quantum mechanics , gene , magnetic field , engineering , immunology
Glycoproteins are crucial in massive physiological events and clinical application. It is necessary to prepare new materials to isolate the specific glycoprotein. New and simple core–shell molecularly imprinted polymers were prepared by surface imprinting. The polymers are synthesized with magnetic nanoparticles as the core, water‐soluble dendritic polyethyleneimine as the monomer and the ovalbumin as the template. The prepared imprinted polymers showed thin imprinted shell, biocompatibility and superparamagnetic properties. The resultant materials exhibited fast kinetics, high adsorption capacity, perfect selectivity and reusability. More important, they can absorb the template glycoprotein from the neutral solution and successfully be applied to recognize the ovalbumin from egg white, which means that they can provide an alternate method to isolate glycoprotein from bodily fluids.