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Simultaneous determination and pharmacokinetic study of four phenol compounds in rat plasma by ultra‐high performance liquid chromatography with tandem mass spectrometry after oral administration of Echinacea purpurea extract
Author(s) -
Gan Chunli,
Liu Lu,
Du Yan,
Wang Liqian,
Gao Mingjie,
Wu Lijun,
Yang Chunjuan
Publication year - 2016
Publication title -
journal of separation science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.72
H-Index - 102
eISSN - 1615-9314
pISSN - 1615-9306
DOI - 10.1002/jssc.201600051
Subject(s) - chromatography , chemistry , electrospray ionization , ammonium acetate , chlorogenic acid , selected reaction monitoring , extraction (chemistry) , tandem mass spectrometry , caffeic acid , high performance liquid chromatography , mass spectrometry , ethyl acetate , analyte , pharmacokinetics , liquid chromatography–mass spectrometry , detection limit , medicine , biochemistry , antioxidant
A rapid and sensitive assay based on ultra‐high performance liquid chromatography with electrospray ionization tandem mass spectrometry was established and validated for the simultaneous determination of cichoric acid, chlorogenic acid, quinic acid, and caffeic acid in rat plasma after oral administration of Echinacea purpurea extract using butylparaben as the internal standard. Samples were pretreated by liquid–liquid extraction with ethyl acetate. The separations for analytes were performed on an ACQUITY UPLC HSS C 18 column (1.8 μm 2.1 × 100 mm) using a gradient elution program with acetonitrile/10 mM ammonium acetate (pH 5.6) at a flow rate of 0.3 mL/min. The analytes were detected in multiple reaction monitoring mode with negative electrospray ionization. The lower limit of quantification of each analyte was not higher than 10.85 ng/mL. The relative standard deviation of the intraday and interday precisions was less than 14.69%. The relative errors of accuracies were in the range of –13.80 to 14.91%. The mean recoveries for extraction recovery and matrix effect were higher than 80.79 and 89.98%, respectively. The method validation results demonstrated that the proposed method was sensitive, specific, and reliable, which was successfully applied to the pharmacokinetic study of four components after oral administration of Echinacea purpurea extract.