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Determination of nine volatile N‐nitrosamines in tobacco and smokeless tobacco products by dispersive solid‐phase extraction with gas chromatography and tandem mass spectrometry
Author(s) -
Lv Fang,
Guo Junwei,
Yu Fei,
Zhang Tingting,
Zhang Shimin,
Cui Huapeng,
Liu Xianjun,
Chen Li,
Liu Leiyu,
Liu Shaofeng,
Xie Fuwei
Publication year - 2016
Publication title -
journal of separation science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.72
H-Index - 102
eISSN - 1615-9314
pISSN - 1615-9306
DOI - 10.1002/jssc.201600037
Subject(s) - chemistry , gas chromatography/tandem mass spectrometry , chromatography , solid phase extraction , mass spectrometry , smokeless tobacco , extraction (chemistry) , gas chromatography , tandem mass spectrometry , gas chromatography–mass spectrometry , nitrosamine , detection limit , amine gas treating , carcinogen , tobacco use , population , organic chemistry , demography , sociology
A method was developed for the determination of nine volatile N‐nitrosamines in tobacco and smokeless tobacco products. The targets are N ‐nitrosodimethylamine, N ‐nitrosopyrrolidine, N ‐nitrosopiperidine, N ‐nitrosomorpholine, N ‐nitrosoethylmethylamine, N ‐nitrosodiethylamine, N ‐nitrosodipropylamine, N ‐nitrosobuylmethylmine, and N ‐nitrosodibutylamine. The samples were treated by dispersive solid‐phase extraction using 1 g of primary secondary amine and 0.5 g of carbon and then analyzed by gas chromatography with tandem mass spectrometry with an electron impact ion source. The recoveries for the targets ranged from 84 to 118%, with <16% relative standard deviations at three spiking levels of 0.5, 1.25, and 2.5 ng/g. The limits of detection ranged from 0.03 to 0.15 ng/g. With the use of the proposed method, we detected the presence of six nitrosamines in the range of 0.4–30.7 ng/g. The study demonstrated that the method could be used as a rapid, convenient, and high‐throughput method for N‐nitrosamines analysis in tobacco matrix.