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Preconcentration of valsartan by dispersive liquid–liquid microextraction based on solidification of floating organic drop and its determination in urine sample: Central composite design
Author(s) -
Pebdani Arezou Amiri,
Shabani Ali Mohammad Haji,
Dadfarnia Shayesteh,
Talebianpoor Mohammad Sharif,
Khodadoust Saeid
Publication year - 2016
Publication title -
journal of separation science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.72
H-Index - 102
eISSN - 1615-9314
pISSN - 1615-9306
DOI - 10.1002/jssc.201600010
Subject(s) - chromatography , detection limit , central composite design , valsartan , extraction (chemistry) , chemistry , drop (telecommunication) , ionic strength , analytical chemistry (journal) , materials science , response surface methodology , medicine , telecommunications , aqueous solution , computer science , blood pressure , radiology
In this work, a fast, easy, and efficient dispersive liquid–liquid microextraction method based on solidification of floating organic drop followed by high‐performance liquid chromatography with UV detection was developed for the separation/preconcentration and determination of the drug valsartan. Experimental design was applied for the optimization of the effective variables (such as volume of extracting and dispersing solvents, ionic strength, and pH) on the extraction efficiency of valsartan from urine samples. The optimized values were 250.0 μL ethanol, 65.0 μL 1‐dodecanol, 4.0% w/v NaCl, pH 3.8, 1.0 min extraction time, and 4.0 min centrifugation at 4000 rpm min −1 . The linear response ( r 2 = 0.997) was obtained in the range of 0.013–10.0 μg mL −1 with a limit of detection of 4.0 ng mL −1 and relative standard deviations of less than 5.0 % ( n = 6).